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This book has been designed to meet the course on Biotechnology for graduate and postgraduate students of Indian universities. It presents a balanced and objective description of basic biotechnological techniques applicable in pure culture methods of microorganisms and other organisms of industrial importance. The book describes the techniques of microscopy, staining, chromatography, tracer techniques and spectrophotometer as applied in understanding the principles of biotechnology. The detail of scientific method, living organisms and the groups of microorganisms, my Corrianne, chromosome and disease, enzymes, proteins and nucleic acids, and carbohydrates have also been dealt with using appropriate examples in this book. The book is useful for graduates and postgraduates in biological sciences studying biotechnology as a stand-alone unit or as part of a broader course. The book will also be of interest to PhD students beginning work in the areas of biotechnology, researchers, professionals and industry specialist in these areas.

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